Claus-Michael Lehr's Cell Culture Models of Biological Barriers: In vitro Test PDF

By Claus-Michael Lehr

ISBN-10: 0203219937

ISBN-13: 9780203219935

ISBN-10: 0203275039

ISBN-13: 9780203275030

ISBN-10: 0415277248

ISBN-13: 9780415277242

During the last ten years a number of refined in vitro attempt platforms in line with epithelial phone cultures were brought within the box of drug supply. those versions were discovered to be very precious in characterizing the permeability of substances throughout epithelial tissues, and in learning formulations or provider structures for stronger drug supply and stronger absorption. in comparison to in vivo trials on animals or people, telephone tradition types are speedier, easier and price potent, ethically beneficial and, most significantly, they are often extra simply standardized and validated.
This publication offers a pragmatic method of modern mobilephone culture-based in vitro concepts for drug delivery experiences at organic absorption boundaries. it's a useful resource of data for college kids attending graduate classes in this topic and pharmaceutical scientists operating in or in academia.

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Extra resources for Cell Culture Models of Biological Barriers: In vitro Test Systems for Drug Absorption and Delivery

Sample text

Eukaryotic plasma membranes contain large amounts of cholesterol, with up to one molecule for every phospholipid molecule. Cholesterol molecules orient themselves in the bilayer with their hydroxyl groups close to the polar head groups of the phospholipid molecules. Their rigid, plate-like steroid rings interact with – and partly immobilize – those regions of the phospholipid hydrocarbon chain that are closest to the polar head groups, leaving the remainder of the chain flexible. Cholesterol decreases the permeability of the membrane to small hydrophilic molecules and enhances both the flexibility and mechanical stability of the bilayer.

Current opinion is that the claudin family of proteins serve as the primary, but not the sole, molecule that fulfills the functional barrier properties of the TJ strands. , 1998), although very little is known about the functional role of this protein. In addition there are several accessory proteins localized to the cytoplasmic surface of TJs. The zonula occluden (ZO) proteins, ZO-1, ZO-2 and ZO-3 form heterodimers potentially serving as the major molecular scaffold for the TJ network. The ZO complexes (through ZO-1 or ZO-2) form crosslinks between the TJ strands and actin filaments.

In the case of adherent cell cultures the passage or subculture is achieved by, firstly, dissociating the cell–matrix and cell– cell, interactions by briefly exposing the cell cultures to a solution of the protease trypsin and/or the divalent ion (Ca++) chelator, ethylenediamine tetraacetic acid (EDTA). The latter binds Ca++ and, therefore, disrupts key Ca++-dependent adhesion forces between cells, and between cells and the matrix. In the case of suspension cultures, the passage or subculturing process simply involves transfer of an aliquot of the cell suspension to fresh medium.

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Cell Culture Models of Biological Barriers: In vitro Test Systems for Drug Absorption and Delivery by Claus-Michael Lehr


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